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we think it is not for two reasons. glas cap 800 First, the Lightcycler is much faster – it completes a full 40-cycle run in 40 minutes – than plate-based cyclers, which typically completes 96 samples in 2.5 h, so you may run three full runs in the Lightcycler by the time it takes to run a single run in a plate-based cycler. Second, since the Lightcycler is extremely precise, you may not gain any additional information by running duplicates or triplicates. It is simply a waste of reagents, because there is essentially no variation in the results. On plate based cyclers it is a necessity to

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