qPCR instrument: LightCycler®480 II

The LightCycler 480 II instrument is used for performing rapid, accurate polymerase chain reaction (PCR) in combination with real-time, online detection enabling quantification of a target nucleic acid, as well as post-PCR analysis of the amplified nucleic acid by melting curve analysis.

The first model was released in September 2005 by Roche and was another breakthrough in real-time PCR analysis of gene expression and melting curve-based mutation analysis.

The system provides the ideal solution for fast and precise qualitative or quantitative detection of nucleic acids, genotyping, and mutation scanning.

Based on a comprehensive improvement of the Peltier-based technology incorporated in a silver block, the instrument provides extraordinary well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility.

All current probe formats are supported:

• SYBR Green I;
• ResoLight dye for high-resolution melting;
• hydrolysis probes;
• HybProbe probes;
• SimpleProbe probes;