qPCR instrument: LightCycler®480 II
The LightCycler 480 II instrument is used for performing rapid, accurate polymerase chain reaction (PCR) in combination with real-time, online detection enabling quantification of a target nucleic acid, as well as post-PCR analysis of the amplified nucleic acid by melting curve analysis.
The first model was released in September 2005 by Roche and was another breakthrough in real-time PCR analysis of gene expression and melting curve-based mutation analysis.
The system provides the ideal solution for fast and precise qualitative or quantitative detection of nucleic acids, genotyping, and mutation scanning.
Based on a comprehensive improvement of the Peltier-based technology incorporated in a silver block, the instrument provides extraordinary well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility.
All current probe formats are supported:
- SYBR Green I;
- ResoLight dye for high-resolution melting;
- hydrolysis probes;
- HybProbe probes;
- SimpleProbe probes;
|Customer number (from supplier)||05015278001|
|Sample Runtime||96 wells in < 1 hour384 wells in < 40 min|
|Sample size||96-well PCR plate: 10 – 100 μl; 384-well PCR plate: 5 – 20 μl;|
|Block format(s)||384 well plate, 96 well plate|
|Number of channels||6|
|Heating and cooling method||Peltier|
|Excitation Source||Xenon lamp|
|Range of excitation/emission wavelengths||440, 465, 498, 533, 618 Detection(nm) : 488, 510, 580, 610,640, 660|
|Include HRM module?||No|
|Life science research only ?||Yes|
qPCR instrument: LightCycler ® 480
Roche LightCycler® 480 System was relased in november 2012 at American Society for Human Genetics...
|Last update: 20-07-2013 20:21||