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  • 9. Protocols

    July 29, 2013

    9.1 Isolation of RNA 9.2 Quantification of RNA by 260/280 nm spectrophotometry 9.3 cDNA synthesis from 0,5 µg of total RNA 9.4 Run real-time PCR reactions; include standard curve 9.5 How to make a standard 9.6 Normalise results relative to housekeeping gene 9.7 Delta Ct or standard curve? 9.8 Should you DNAse treat your samples? Read more…

    • Posted in PCR Principle (Tutorial)
    • Tags: Pcr method, Pcr troubleshooting, Quantitative pcr tutorial, Real time pcr tutorial
    • By knud josefsen
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