9.1 Isolation of RNA 9.2 Quantification of RNA by 260/280 nm spectrophotometry 9.3 cDNA synthesis from 0,5 µg of total RNA 9.4 Run real-time PCR reactions; include standard curve 9.5 How to make a standard 9.6 Normalise results relative to housekeeping gene 9.7 Delta Ct or standard curve? 9.8 Should you DNAse treat your samples? Read more…