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2 Comments

  • Hanne Hansen | March 4, 2014 at 12:09 pm

    What is the issue of DNAse treatment?
    Thank you!

    • Knud Josefsen | March 5, 2014 at 2:14 am

      Usually, we don’t use DNAse treatment. The test is just to add RNA that has not been reverse transcribed. You should not get any amplification.
      One situation where you would need it, though, is if you are monitoring the efficiency of transformations. Even if DNA is not supposed to be isolated during the isolation of RNA, there is always some left.

      Sincerely,

      Knud

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