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knud josefsen | Real Time PCR - Part 9
July 28, 2013

The result of a real time PCR analysis is an expression value of your target gene of interest. However, if the amount of cDNA which was used for the real time PCR reaction varies, i.e. due to an inaccurate quantification of your RNA used for the cDNA analysis or variable cDNA synthesis efficiencies, your result Read more…